State of the Gingival Sulcus Microbiome in Children with Bronchial Asthma
https://doi.org/10.31550/1727-2378-2024-23-3-42-48
Abstract
Aim. To compare the microbiome of the healthy gingival sulcus of children with bronchial asthma (BA) and different dental conditions.
Design. Open comparative clinical and laboratory study.
Materials and methods. Gingival substrate was obtained from 19 children aged 3–6 years with exacerbation of BA; the results of sequencing the genes of 16 subunits of ribosomal ribonucleic acid (16S rRNA) on the MiSeq platform (Illumina) were analyzed.
Results. Five major phyla have been identified in the gingival sulcus: Firmicutes, Proteobacteria, Actinobacteria, Fusobacterium and Bacteroidetes and the dominant genus, Streptococcus. A significantly high relative prevalence of the species Neisseria oralis (p = 0.0073), Hemophilic massiliensis (p = 0.046), Hemophilic paraphrohaemolyticus (p = 0.05) and Lautropia mirabilis (p = 0.05) in children with asthma without caries was established. The level of immunoglobulin E (IgE) is significantly higher in children with asthma and caries compared to children without caries (461.79 ± 60.58 and 276.97 ± 81.15 IU/ml).
Conclusion. The predominance of opportunistic and “healthy” bacterial species of the phylum Proteobacteria in the healthy gingival sulcus of children with BA may be an indicator of oral dysbiosis in BA. Overproduction of IgE in children with caries may be due to cariogenic flora, which requires further study.
About the Authors
T. V. Vivtanenko
Orenburg State Medical University
Russian Federation
Russian Federation
6 Sovetskaya Str., Orenburg, 460000
L. Yu. Popova
Orenburg State Medical University
Russian Federation
Russian Federation
6 Sovetskaya Str., Orenburg, 460000
Yu. A. Khlopko
Institute of Cellular and Intracellular Symbiosis, Ural Branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
11 Pionerskaya Str., Orenburg, 460000
R. R. Demina
Orenburg State Medical University
Russian Federation
Russian Federation
6 Sovetskaya Str., Orenburg, 460000
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Review
For citations:
Vivtanenko T.V., Popova L.Yu., Khlopko Yu.A., Demina R.R. State of the Gingival Sulcus Microbiome in Children with Bronchial Asthma. Title. 2024;23(3):42-48. (In Russ.) https://doi.org/10.31550/1727-2378-2024-23-3-42-48
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