docruДоктор.РуTitle1727-23782713-2994ООО "ГК "РУСМЕДИКАЛ"10.31550/1727-2378-2024-23-3-42-48docru-147Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL PAPERSСостояние микробиома десневой борозды детей с бронхиальной астмойState of the Gingival Sulcus Microbiome in Children with Bronchial Asthmahttps://orcid.org/0009-0005-0943-0896ВивтаненкоТ. В.VivtanenkoT. V.

Вивтаненко Татьяна Владимировна — к. м. н., доцент кафедры детских болезней

460000, г. Оренбург, ул. Советская, д. 6

6 Sovetskaya Str., Orenburg, 460000

tatwiwt@mail.ruhttps://orcid.org/0000-0001-6306-7104ПоповаЛ. Ю.PopovaL. Yu.

Попова Лариса Юрьевна — д. м. н., профессор, заведующая кафедрой детских болезней

460000, г. Оренбург, ул. Советская, д. 6

6 Sovetskaya Str., Orenburg, 460000

docpopova@yandex.ruhttps://orcid.org/0000-0002-2880-3214ХлопкоЮ. А.KhlopkoYu. A.

Хлопко Юрий Александрович  — д. т. н., доцент, ведущий научный сотрудник

460000, Оренбург, ул. Пионерская, д. 11

11 Pionerskaya Str., Orenburg, 460000

140374@mail.ruhttps://orcid.org/0000-0002-0697-6165ДеминаР. Р.DeminaR. R.

Демина Римма Раильевна — к. м. н., доцент кафедры терапевтической стоматологии

460000, г. Оренбург, ул. Советская, д. 6

6 Sovetskaya Str., Orenburg, 460000

deminarr@mail.ruФГБОУ ВО «Оренбургский государственный медицинский университет» Минздрава РоссииРоссияOrenburg State Medical UniversityRussian FederationФГБУН «Институт клеточного и внутриклеточного симбиоза» УрО РАНРоссияInstitute of Cellular and Intracellular Symbiosis, Ural Branch of the Russian Academy of SciencesRussian Federation202417022025233ПЕДИАТРИЯ4248Copyright © Вивтаненко Т.В., Попова Л.Ю., Хлопко Ю.А., Демина Р.Р., 20252025Вивтаненко Т.В., Попова Л.Ю., Хлопко Ю.А., Демина Р.Р.Vivtanenko T.V., Popova L.Y., Khlopko Y.A., Demina R.R.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://docru.elpub.ru/jour/article/view/147Цель исследования

Цель исследования: сравнить микробиом здоровой десневой борозды детей с бронхиальной астмой (БА) при различном состоянии зубов.

Дизайн

Дизайн: открытое сравнительное клинико-лабораторное исследование.

Материалы и методы

Материалы и методы. Десневой субстрат получен у 19 детей 3–6 лет с обострением БА, проанализированы результаты секвенирования генов 16 субъединиц рибосомной рибонуклеиновой кислоты (16S рРНК) на платформе «MiSeq» («Illumina»).

Результаты

Результаты. Определены 5 основных филумов в десневой борозде: Firmicutes, Proteobacteria, Actinobacteria, Fusobacterium и Bacteroidetes и доминирующий род — Streptococcus. Установлена достоверно высокая относительная распространенность видов Neisseria oralis (p = 0,0073), Hemophilic massiliensis (p = 0,046), Hemophilic paraphrohaemolyticus (p = 0,05) и Lautropia mirabilis (p = 0,05) у детей с БА без кариеса. Уровень иммуноглобулина Е (IgЕ) достоверно выше у детей с астмой и кариесом в сравнении с детьми без кариеса (461,79 ± 60,58 и 276,97 ± 81,15 МЕ/мл).

Заключение

Заключение. Преобладание условно-патогенных и «здоровых» бактериальных видов филума Proteobacteria в здоровой десневой борозде детей с БА может являться показателем дисбиоза ротовой полости при БА. Гиперпродуция IgЕ у детей с кариесом, возможно, обусловлена кариесогенной флорой, что требует дальнейшего изучения.

Aim

Aim. To compare the microbiome of the healthy gingival sulcus of children with bronchial asthma (BA) and different dental conditions.

Design

Design. Open comparative clinical and laboratory study.

Materials and methods

Materials and methods. Gingival substrate was obtained from 19 children aged 3–6 years with exacerbation of BA; the results of sequencing the genes of 16 subunits of ribosomal ribonucleic acid (16S rRNA) on the MiSeq platform (Illumina) were analyzed.

Results

Results. Five major phyla have been identified in the gingival sulcus: Firmicutes, Proteobacteria, Actinobacteria, Fusobacterium and Bacteroidetes and the dominant genus, Streptococcus. A significantly high relative prevalence of the species Neisseria oralis (p = 0.0073), Hemophilic massiliensis (p = 0.046), Hemophilic paraphrohaemolyticus (p = 0.05) and Lautropia mirabilis (p = 0.05) in children with asthma without caries was established. The level of immunoglobulin E (IgE) is significantly higher in children with asthma and caries compared to children without caries (461.79 ± 60.58 and 276.97 ± 81.15 IU/ml).

Conclusion

Conclusion. The predominance of opportunistic and “healthy” bacterial species of the phylum Proteobacteria in the healthy gingival sulcus of children with BA may be an indicator of oral dysbiosis in BA. Overproduction of IgE in children with caries may be due to cariogenic flora, which requires further study.

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The authors declare that there are no conflicts of interest present.